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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in lots of tumors, which leads to chromosome instability, and so may possibly lead to tumorigenesis. This protein was located to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a critical function in marketing mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been explained. Two additional splice variants had been described but in depth nucleotide sequence information and facts is not really nevertheless offered.
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Blocking peptides are peptides that bind exclusively to your focus on antibody and block antibody binding. These peptide typically is made up of the epitope acknowledged with the antibody.
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This information outlines even more doable optimization of mobile and immunoassay parameters to be certain the best possible success are...
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This tutorial outlines additional achievable optimization of mobile and immunoassay parameters to make sure the very best success are...
Antibodies bound to the blocking peptide no more bind for the epitope over the concentrate on protein. By comparing the staining through the blocked antibody versus the antibody by yourself, you can see which staining is specific.
Goal Facts Cyclin E belongs to the remarkably conserved cyclin family members, whose associates are characterised by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation designs which add to your temporal coordination of each and every mitotic function. Cyclin E types a complex with and functions being a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S transition. This protein accumulates for the G1-S section boundary and is particularly degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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